کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4337880 1614825 2013 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Methods of dendritic spine detection: From Golgi to high-resolution optical imaging
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Methods of dendritic spine detection: From Golgi to high-resolution optical imaging
چکیده انگلیسی

Dendritic spines, the bulbous protrusions that form the postsynaptic half of excitatory synapses, are one of the most prominent features of neurons and have been imaged and studied for over a century. In that time, changes in the number and morphology of dendritic spines have been correlated to the developmental process as well as the pathophysiology of a number of neurodegenerative diseases. Due to the sheer scale of synaptic connectivity in the brain, work to date has merely scratched the surface in the study of normal spine function and pathology. This review will highlight traditional approaches to the imaging of dendritic spines and newer approaches made possible by advances in microscopy, protein engineering, and image analysis. The review will also describe recent work that is leading researchers toward the possibility of a systematic and comprehensive study of spine anatomy throughout the brain.


► Golgi staining and manual spine identification have provided insight into neurodegeneration.
► Advances in microscopy and tissue labeling have allowed in vivo studies in animals.
► Automated spine algorithms are necessary tools for a system-level scale study.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 251, 22 October 2013, Pages 129–140
نویسندگان
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