کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4337965 | 1614835 | 2013 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: IGF-I redirects doublecortin-positive cell migration in the normal adult rat brain IGF-I redirects doublecortin-positive cell migration in the normal adult rat brain](/preview/png/4337965.png)
• Ectopic IGF-1 redirects the migration of Dcx-positive cells into the normal striatum.
• IGF-I is an inducer of migration and acts by both chemotaxis and chemokinesis.
• Ectopic IGF-1 had no effect on cell proliferation or neuronal differentiation.
The migration of subventricular zone (SVZ)-derived neural precursor cells through the rostral migratory stream (RMS) to the olfactory bulb is tightly regulated by local micro-environmental cues. Insulin-like Growth Factor-I (IGF-I) can stimulate the migration of several neuronal cell types and acts as a ‘departure’ factor in the avian SVZ. To establish whether IGF-I can also act as a migratory factor for adult neuronal precursor cells in vivo, in addition to its well established role in precursor cell proliferation and differentiation, we used AAV2-mediated gene transfer to produce ectopic expression of IGF-I in the normal adult rat striatum. We then assessed whether the expression of IGF-I would recruit SVZ-derived neuronal precursor cells from the RMS into the striatum. Ectopic expression of IGF-I in the normal adult rat brain significantly increased the number of doublecortin (Dcx)-positive cells and the extent of their migration into the striatum 4 and 8 weeks after AAV2–IGF-I injection but did not promote neuronal differentiation. In vitro migration assays confirmed that IGF-I is an inducer of migration and directs SVZ-derived adult neuronal precursor cell migration by both chemotaxis and chemokinesis. These results demonstrate that overexpression of IGF-I in the normal adult rat brain can override the normal cues directing precursor cell migration along the RMS and can redirect precursor cell migration into a non-neurogenic region. Enhanced expression of IGF-I following brain injury may therefore act as a diffusible factor mediating precursor cell migration to areas of neuronal cell damage.
Journal: Neuroscience - Volume 241, 25 June 2013, Pages 106–115