کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4337965 1614835 2013 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
IGF-I redirects doublecortin-positive cell migration in the normal adult rat brain
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
IGF-I redirects doublecortin-positive cell migration in the normal adult rat brain
چکیده انگلیسی


• Ectopic IGF-1 redirects the migration of Dcx-positive cells into the normal striatum.
• IGF-I is an inducer of migration and acts by both chemotaxis and chemokinesis.
• Ectopic IGF-1 had no effect on cell proliferation or neuronal differentiation.

The migration of subventricular zone (SVZ)-derived neural precursor cells through the rostral migratory stream (RMS) to the olfactory bulb is tightly regulated by local micro-environmental cues. Insulin-like Growth Factor-I (IGF-I) can stimulate the migration of several neuronal cell types and acts as a ‘departure’ factor in the avian SVZ. To establish whether IGF-I can also act as a migratory factor for adult neuronal precursor cells in vivo, in addition to its well established role in precursor cell proliferation and differentiation, we used AAV2-mediated gene transfer to produce ectopic expression of IGF-I in the normal adult rat striatum. We then assessed whether the expression of IGF-I would recruit SVZ-derived neuronal precursor cells from the RMS into the striatum. Ectopic expression of IGF-I in the normal adult rat brain significantly increased the number of doublecortin (Dcx)-positive cells and the extent of their migration into the striatum 4 and 8 weeks after AAV2–IGF-I injection but did not promote neuronal differentiation. In vitro migration assays confirmed that IGF-I is an inducer of migration and directs SVZ-derived adult neuronal precursor cell migration by both chemotaxis and chemokinesis. These results demonstrate that overexpression of IGF-I in the normal adult rat brain can override the normal cues directing precursor cell migration along the RMS and can redirect precursor cell migration into a non-neurogenic region. Enhanced expression of IGF-I following brain injury may therefore act as a diffusible factor mediating precursor cell migration to areas of neuronal cell damage.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 241, 25 June 2013, Pages 106–115
نویسندگان
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