Saffron extract and trans-crocetin inhibit glutamatergic synaptic transmission in rat cortical brain slices

Saffron, the dried stigmata of Crocus sativus L., is used in traditional medicine for a wide range of indications including cramps, asthma, and depression. To investigate the influence of hydro-ethanolic saffron extract (CSE) and trans-crocetin on synaptic transmission, postsynaptic potentials (PSPs) were elicited by focal electrical stimulation and recorded using intracellular placed microelectrodes in pyramidal cells from rat cingulate cortex. CSE (10–200 μg/ml) inhibited evoked PSPs as well as the isolated NMDA and non-NMDA component of PSPs. Glutamate (500 μM) added into the organ bath induced membrane depolarization. CSE decreased glutamate-induced membrane depolarization. Additionally, CSE at 100 μg/ml decreased NMDA (20 μM) and kainate (1 μM)-induced depolarization, whereas AMPA (1 μM)-induced depolarization was not affected. Trans-crocetin (1–50 μM) showed inhibition of evoked PSPs and glutamate-induced membrane depolarization comparable to CSE. Trans-crocetin at 10 μM decreased NMDA (20 μM)-induced membrane depolarization, but did not inhibit the isolated non-NMDA component of PSPs. We conclude that trans-crocetin is involved in the antagonistic effect of CSE on NMDA but not on kainate receptors.

▶Saffron and trans-crocetin decreased the glutamate-induced membrane depolarization. ▶Saffron and trans-crocetin inhibited synaptic transmission in rat pyramidal cells. ▶Mechanism—antagonism on NMDA and kainate receptors. ▶Trans-crocetin antagonized NMDA but not kainate receptors.