کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4339100 1614898 2011 22 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Vesicular glutamate transporters: spatio-temporal plasticity following hearing loss
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Vesicular glutamate transporters: spatio-temporal plasticity following hearing loss
چکیده انگلیسی

An immunocytochemical comparison of vGluT1 and vGluT3 in the cochlear nucleus (CN) of deafened versus normal hearing rats showed the first example of vGluT3 immunostaining in the dorsal and ventral CN and revealed temporal and spatial changes in vGluT1 localization in the CN after cochlear injury. In normal hearing rats vGluT1 immunostaining was restricted to terminals on CN neurons while vGluT3 immunolabeled the somata of the neurons. This changed in the ventral cochlear nucleus (VCN) 3 days following deafness, where vGluT1 immunostaining was no longer seen in large auditory nerve terminals but was instead found in somata of VCN neurons. In the dorsal cochlear nucleus (DCN), while vGluT1 labeling of terminals decreased, there was no labeling of neuronal somata. Therefore, loss of peripheral excitatory input results in co-localization of vGluT1 and vGluT3 in VCN neuronal somata. Postsynaptic glutamatergic neurons can use retrograde signaling to control their presynaptic inputs and these results suggest vGluTs could play a role in regulating retrograde signaling in the CN under different conditions of excitatory input. Changes in vGluT gene expression in CN neurons were found 3 weeks following deafness using qRT-PCR with significant increases in vGluT1 gene expression in both ventral and dorsal CN while vGluT3 gene expression decreased in VCN but increased in DCN.

Research highlights▶vGluT3 is expressed and produced in the cochlear nucleus. ▶Activity level governs neuronal compartmentalization of vGluTs. ▶Plasticity dependent co-localization of vGluTs occurs after hearing loss. ▶Activity dependent somatic localization of vGluTs suggests retrograde signaling.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 178, 31 March 2011, Pages 218–239
نویسندگان
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