کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4341126 | 1295824 | 2007 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Two types of neurotransmitter release patterns in isolectin B4-positive and negative trigeminal ganglion neurons
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کلمات کلیدی
IB4small synaptic vesicleFM4-64SSVTRGLDCVCGRPTTXFBSHBSSNGFEndocytosis - آندوسیتوز یا درون بریExocytosis - اگزوسیتوزisolectin B4 - ایزوکتین B4tetrodotoxin - تترو دوتوکسین Fluorescent dye - رنگ فلورسنتfetal bovine serum - سرم جنین گاوnerve growth factor - فاکتور رشد عصبHanks’ balanced salt solution - محلول نمک متعادل هانکسNociceptor - مسکنSeries resistance - مقاومت سریMembrane resistance - مقاومت غشاءSensory neurons - نورونهای حسیcalcitonin gene-related peptide - پپتید مرتبط با ژن کلسی تونینtrigeminal ganglion - گانگلیون سه گانه
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Two types of neurotransmitter release patterns in isolectin B4-positive and negative trigeminal ganglion neurons Two types of neurotransmitter release patterns in isolectin B4-positive and negative trigeminal ganglion neurons](/preview/png/4341126.png)
چکیده انگلیسی
Mammalian nociceptors have been classified into subclasses based on differential neurotrophin sensitivity and binding of the plant isolectin B4 (IB4). Most of the nerve growth factor-responsive IB4-negative (IB4 (â)) nociceptors contain neuropeptides such as substance P and calcitonin gene-related peptide, whereas the glial-derived neurotrophic factor-responsive IB4-positive (IB4 (+)) neurons predominantly lack such neuropeptides. We hypothesized that the differences in neuropeptide content between IB4 (+) and (â) neurons might be reflected in differences in stimulated exocytosis and/or endocytosis. To address this, we monitored the secretory activity of acutely dissociated neurons from adult rat trigeminal ganglia (TRG) using cell membrane capacitance (Cm) measurements and the fluorescent membrane-uptake marker N-(3-triethylammoniumpropyl)-4-(6-(4-(diethylamino)phenyl)hexatrienyl)pyridinium dibromide (FM4-64). Cm measurements were performed under whole-cell voltage clamp and neurons were depolarized from â75 mV to +10 mV to elicit exocytosis. Both types of TRG neurons showed similarly-sized, calcium-dependent increases in Cm, demonstrating that both IB4 (+) and (â) TRG neurons are capable of stimulated exocytosis. However, the peak Cm of IB4 (+) neurons decayed faster toward baseline than that of IB4 (â) neurons. Also, IB4 (+) neurons had stable Cm responses to repeated stimuli whereas IB4 (â) neurons loss their secretory response during repeated stimulation. These data suggested that the IB4 (+) neurons possess a faster rate of endocytosis and vesicle replenishment than IB4 (â) neurons. To test this, we measured vesicle trafficking with the fluorescent membrane dye FM4-64. FM4-64 staining showed that IB4 (â) neurons exhibit a larger pool of endocytosed vesicles than IB4 (+) neurons because the peak fluorescence increases in IB4 (â) neurons were larger but slower than in IB4 (+) neurons. However, the recycled vesicles were released faster in IB4 (+) compared with IB4 (â) neurons. Taken together these data suggest that the IB4 (+) TRG neurons have faster exocytosis and endocytosis than the IB4 (â) neurons.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 144, Issue 2, 19 January 2007, Pages 665-674
Journal: Neuroscience - Volume 144, Issue 2, 19 January 2007, Pages 665-674
نویسندگان
Y. Matsuka, B. Edmonds, S. Mitrirattanakul, F.E. Schweizer, I. Spigelman,