کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4341748 1295845 2006 18 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular identification of an N-type Ca2+ channel in saccular hair cells
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Molecular identification of an N-type Ca2+ channel in saccular hair cells
چکیده انگلیسی

We report new molecular evidence for the presence of an N-type (Cav2.2, α1B) voltage-gated Ca2+ channel in hair cells of the saccular epithelium of the rainbow trout. The Cav2.2 amino-acid sequence shows 68% and 63% identity compared with chick and human Cav2.2, respectively. This channel reveals features that are characteristic of an N-type Ca2+ channel: an ω-conotoxin GVIA binding domain, Gβγ binding regions, and a synaptic protein interaction site. Immunohistochemical studies with a custom antibody show that immunoreactivity for the Cav2.2 is concentrated in the basolateral and apical regions of hair cells. Whereas trout brain and saccular macula express an 11-amino-acid insert in the second Gβγ binding domain of the Cav2.2 I–II loop, isolated hair cells appear not to express this variant. We constructed fusion polypeptides representing portions of the I–II loop, β1 and β2a auxiliary subunits, the II–III loop, and syntaxin, and examined their intermolecular interactions via immunoprecipitation and surface plasmon resonance. The I–II loop polypeptides bound both β1 and β2a subunits with a preference for β1, and the II–III loop exhibited Ca2+-dependent syntaxin binding. We demonstrated syntaxin immunoreactivity near afferent endings in hair cells, at hair-cell apices, and in efferent endings on hair cells, the former two sites consistent with binding of syntaxin to Cav2.2. The present molecular characterization of the Cav2.2 channel provides novel biochemical evidence for an N-type channel in hair cells, and details molecular interactions of this channel that reflect hair-cell function, such as spontaneous activity and vesicular trafficking. The current work, to our knowledge, represents the first demonstration of a putative N-type channel in hair cells as documented by tissue-specific antibody immunoreactivity and hair-cell-specific cDNA sequence.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 139, Issue 4, 2006, Pages 1417–1434
نویسندگان
, , , , , , ,