کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4341919 | 1295850 | 2006 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Distribution and expression pattern of the nitrergic system in the cerebellum of the sheep
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کلمات کلیدی
PBSUBCnNOSiNOSNADPHcGMPNOSUnipolar brush cell - سلول برس یونیپلارinducible nitric oxide synthase - سنتاز اکسید نیتریک القاییneuronal nitric oxide synthase - سنتاز اکسید نیتریک عصبیPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریCerebellum - مخچهnitrotyrosine - نیتروتیروستینNitric oxide - نیتریک اکسیدnitric oxide synthase - نیتریک اکسید سنتازnicotinamide adenine dinucleotide phosphate - نیکوتین آمید adenine dinucleotide phosphateSheep - گوسفند
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
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چکیده انگلیسی
The nitrergic system produces nitric oxide as an atypical neurotransmitter in the nervous system. Nitric oxide is produced from l-arginine through specific enzymes known as nitric oxide synthases. Of these, the more abundant form in neurons is the constitutive neuronal nitric oxide synthase, although the inducible isoform can be expressed as well, especially following stress or other injuries. The excessive formation of nitric oxide results in protein nitration, particularly at tyrosine residues, thus the presence of nitrotyrosine can be used as a marker of nitric oxide production. In previous studies we have shown the distribution of the components of the nitrergic system in the cerebellum of rodents, where neuronal nitric oxide synthase immunoreactivity was present in stellate and basket cells, and occasionally in granule cells. Here, we present evidence that in the sheep, as a model of larger mammals, most cerebellar neurons display an intense immunostaining for neuronal nitric oxide synthase, including unipolar brush cells, and Lugaro and Golgi neurons, which are not immunoreactive in rodents. In addition, weak immunoreactivity for inducible nitric oxide synthase and nitrotyrosine was found in particular cell types, indicating a basal expression for these markers. Our results suggest a larger dependence on the nitrergic system for the cerebella of larger mammals. Since this increase happens in both activating and inhibitory neurons of the cerebellar circuitry, we propose that in these animals there is a higher steady-state regulation of the cerebellum based on nitric oxide.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 139, Issue 3, 2006, Pages 889-898
Journal: Neuroscience - Volume 139, Issue 3, 2006, Pages 889-898
نویسندگان
J. Rodrigo, A.P. Fernández, J. Serrano, M. Monzón, E. Monleón, J.J. Badiola, S. Climent, R. MartÃnez-Murillo, A. MartÃnez,