کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4342165 | 1295858 | 2007 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Significant photoreceptor rescue by treatment with a combination of antioxidants in an animal model for retinal degeneration
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کلمات کلیدی
PBSReduced l-glutathioneH/ERD1rd1 mousePDE6GSHAMDALA8-OHdGRetinitis pigmentosaTrxONLINL8-hydroxy deoxyguanosine - 8-هیدروکسی دگزیهوانوزینDMSO - DMSOAntioxidants - آنتی اکسیدانAlpha lipoic acid - اسید لیپوئیک آلفاTerminal deoxynucleotidyl transferase-mediated dUTP nick end labeling - ترمینال deoxynucleotidyl transferase-mediated dUTP نام نهایی پایان نامهNeurodegeneration - تولید نوروژنیکTUNEL - تونلthioredoxin - تیرودوکسینretinal degeneration - دژنراسیون شبکیهPhotoreceptor degeneration - دژنراسیون فتوترپتورDimethylsulfoxide - دیمتیل سولفواکسیدpostnatal day - روز پس از زایمانage-related macular degeneration - سن تخریب ماکولا مربوط به سن استouter nuclear layer - لایه بیرونی هسته ایinner nuclear layer - لایه داخلی هسته ایPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریwild type - نوع وحشیhematoxylin/eosin - هماتوکسیلین / ائوزینReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
The purpose of this study was to investigate the presence of oxidative DNA damage in the photoreceptors of the rd1 mouse, an animal model for retinitis pigmentosa, and to determine if antioxidants could delay the progress of photoreceptor cell death. Retinas of rd1 mice and congenic wild type controls were examined for DNA oxidation and fragmentation. To study the rescue effect of antioxidants on retinal degeneration, rd1 retinas were studied in vitro and in vivo using lutein, zeaxanthin, alpha lipoic acid and reduced l-glutathione. For the in vitro studies, antioxidants were added to the culture medium. For the in vivo studies, postnatal day (PN3) pups of rd1 mice were fed antioxidants either individually or in combination and control rd1 animals received vehicle alone. Histological evaluation was performed using hematoxylin/eosin and avidin staining, as well as terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. Many of the rd1 rod photoreceptors at PN11 displayed oxidative DNA damage and TUNEL positive reaction which co-localized in a subset of rod photoreceptors. Avidin-labeled rod photoreceptors were more abundant than the TUNEL positive photoreceptors of the rd1 mouse, indicating that oxidative DNA damage precedes fragmentation. The number of TUNEL positive and avidin positive cells was considerably decreased upon treatment with the combination of the antioxidants. Rescue of rd1 photoreceptors was significant at PN18 and PN17, respectively, in the in vitro and in vivo studies. In conclusion individual antioxidants had no significant rescue effect but the combination slowed down the rd1 rod photoreceptor degeneration, indicating an additive or synergistic effect.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 145, Issue 3, 30 March 2007, Pages 1120-1129
Journal: Neuroscience - Volume 145, Issue 3, 30 March 2007, Pages 1120-1129
نویسندگان
M.M. Sanz, L.E. Johnson, S. Ahuja, P.A.R. Ekström, J. Romero, T. van Veen,