کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4351515 | 1298058 | 2012 | 7 صفحه PDF | دانلود رایگان |
Reporters of Cre and/or Flp activity are important for defining the spatial and temporal extent of Cre/Flp-mediated recombination. Here, we describe R26-CAG-LF-mTFP1, a multifunctional fluorescent reporter mouse that strongly expresses mTFP1 (bright teal fluorescent protein) after Cre- and Flp-mediated recombination. To meet the need for single recombinase-mediated reporter expression, we generated derivatives of R26-CAG-LF-mTFP1. The germline excision of the Frt-flanked stop cassette in R26-CAG-LF-mTFP1 generated a Cre-dependent reporter (R26-CAG-LoxP-mTFP1). Similarly, R26-CAG-FRT-mTFP1, in which the loxP-flanked stop cassette was excised in the germline, requires only Flp to activate mTFP1 expression.
► We generated a new Cre/Flp fluorescent reporter mouse strain (R26-CAG-LF-mTFP1).
► To meet the need for single recombinase-mediated reporter expression, we generated derivatives of R26-CAG-LF-mTFP1.
► We evaluated these reporter mouse strains in the central nervous system.
Journal: Neuroscience Research - Volume 73, Issue 1, May 2012, Pages 85–91