کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4351526 | 1298059 | 2013 | 6 صفحه PDF | دانلود رایگان |
Temporally precise inhibition of genetically defined cell populations in intact nervous systems has been enabled by the microbial halorhodopsin NpHR, a fast, light-activated chloride pump. Here, we report the generation of new mouse strains that express eNpHR2-EYFP fusion proteins after Cre- and/or Flp-mediated recombination to silence neural activity in vivo. In these mouse strains, Cre/Flp recombination induced a high-level of eNpHR2-EYFP expression. Slice whole-cell patch clamp experiments confirmed that eNpHR2-EYFP-expressing neurons could be optically hyperpolarized and inhibited from firing action potentials. Thus, these mouse strains offer powerful tools for light-induced silencing of neural activity in genetically defined cell populations.
► Light-induced silencing of neural activity in genetically defined cell populations.
► We generated new mouse strains that express eNpHR2-EYFP fusion proteins.
► In these mice, Cre/Flp recombination induced a high-level of eNpHR2-EYFP expression.
Journal: Neuroscience Research - Volume 75, Issue 1, January 2013, Pages 53–58