کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4351529 1298059 2013 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Targeted expression of a chimeric channelrhodopsin in zebrafish under regulation of Gal4-UAS system
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Targeted expression of a chimeric channelrhodopsin in zebrafish under regulation of Gal4-UAS system
چکیده انگلیسی

Channelrhodopsin (ChR)-wide receiver (ChRWR), one of the chimeric molecule of ChR1 and ChR2, has several advantages over ChR2 such as improved expression in the plasma membrane and enhanced photocurrent with small desensitization. Here we generated transgenic zebrafish (Danio rerio) expressing ChRWR as a conjugate of EGFP under the regulation of UAS promoter (UAS:ChRWR-EGFP). When crossed with a Gal4 line, SAGFF36B, ChRWR-EGFP was selectively expressed in primary mechanosensory Rohon–Beard (RB) neurons. The direct photoactivation of RB neurons was sufficient to trigger the escape behavior. The UAS:ChRWR-EGFP line could facilitate a variety of investigations of neural networks and behaviors of zebrafish in vivo.


► ChRWR is optimized for stimulating a neuron with low intensity of light.
► We generated UAS:ChRWR-EGFP line of zebrafish expressing ChRWR under UAS promoter.
► When crossed with an SAGFF36B line, ChRWR was expressed in Rohon–Beard neurons.
► The escape behavior was elicited by photoactivation of Rohon–Beard neurons.
► The UAS:ChRWR-EGFP line is an useful tool to study neuronal network of zebrafish.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Research - Volume 75, Issue 1, January 2013, Pages 69–75
نویسندگان
, , , , , , ,