کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4353171 | 1298201 | 2007 | 11 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: T-type Ca2+ channels contribute to IBMX/forskolin- and K+-induced Ca2+ transients in porcine olfactory receptor neurons T-type Ca2+ channels contribute to IBMX/forskolin- and K+-induced Ca2+ transients in porcine olfactory receptor neurons](/preview/png/4353171.png)
T-type Ca2+ channels are low-voltage-activated Ca2+ channels that control Ca2+ entry in excitable cells during small depolarization above resting potentials. Using Ca2+ imaging with a laser scanning confocal microscope we investigated the involvement of T-type Ca2+ channels in IBMX/forskolin- and sparingly elevated extracellular K+-induced Ca2+ transients in freshly isolated porcine olfactory receptor neurons (ORNs). In the presence of mibefradil (10 μM) or Ni2+ (100 μM), the selective T-type Ca2+ channel inhibitors, IBMX/forskolin-induced Ca2+ transients in the soma were either strongly (>60%) inhibited or abolished completely. However, the Ca2+ transients in the knob were only partially (<60%) inhibited. Ca2+ transients induced by 30 mM K+ were also partially (∼60%) inhibited at both the knob and soma. Furthermore, ORNs responded to as little as a 2.5 mM increase in the extracellular K+ concentration (7.5 mM K+), and such responses were completely inhibited by mibefradil or Ni2+. These results reveal functional expression of T-type Ca2+ channels in porcine ORNs, and suggest a role for these channels in the spread Ca2+ transients from the knob to the soma during activation of the cAMP cascade following odorant binding to G-protein-coupled receptors on the cilia/knob of ORNs.
Journal: Neuroscience Research - Volume 57, Issue 1, January 2007, Pages 129–139