کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4356734 1299990 2016 16 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterizing the nuclear proteome of Paracoccidioides spp.
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
Characterizing the nuclear proteome of Paracoccidioides spp.
چکیده انگلیسی


• We provide the first description of the nuclear proteins in species of Paracoccidioides.
• The Paracoccidioides species, Paracoccidioides lutzii and Paracoccidioides brasiliensis, were investigated.
• Paracoccidioides nuclear proteome reveals a different profile between the species.

Paracoccidioidomycosis is an endemic disease in Latin America, caused by thermo dimorphic fungi of the genus Paracoccidioides. Although previous proteome analyses of Paracoccidioides spp. have been carried out, the nuclear subproteome of this pathogen has not been described. In this way, we aimed to characterize the nuclear proteome of Paracoccidioides species, in the yeast form. For that, yeast cells were disrupted and submitted to cell fractionation. The purity of the nuclear fraction was confirmed by fluorescence and electron microscopy. Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) allowed the identification of 867 proteins. In order to support our enrichment method for nuclear proteins, bioinformatics analysis were applied that allowed the identification of 281 proteins with nuclear localization. The analysis revealed proteins related to DNA maintenance, gene expression, synthesis and processing of messenger and ribosomal RNAs, likewise proteins of nuclear-cytoplasmic traffic. It was also possible to detect some proteins that are poorly expressed, like transcription factors involved in important roles such as resistance to abiotic stress, sporulation, cellular growth and DNA and chromatin maintenance. This is the first descriptive nuclear proteome of Paracoccidioides spp. that can be useful as an important platform base for fungi-specific nuclear processes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fungal Biology - Volume 120, Issue 10, October 2016, Pages 1209–1224
نویسندگان
, , , , , ,