The PaAlr1 magnesium transporter is required for ascospore development in Podospora anserina

The PaAlr1 gene encoding a putative plasma membrane magnesium (Mg) transporter in Podospora anserina was inactivated. The PaAlr1Δ mutants showed sensitivity to deprivation and excess Mg2+ and Ca2+. They also exhibited an autonomous ascospore maturation defect. Mutant ascospores were arrested at an early stage when they contained two nuclei. These data emphasize the role of Mg ions during sexual development in a filamentous fungus.

► The PaAlr1 gene encodes a putative magnesium (Mg) transporter in Podospora anserina.
► The PaAlr1 gene was inactivated.
► Mutants showed sensitivity to deprivation and excess Mg2+.
► Mutants exhibited a specific autonomous ascospore maturation defect.