کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4357761 | 1300104 | 2008 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A family 51 α-l-arabinofuranosidase from Penicillium purpurogenum: purification, properties and amino acid sequence
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A family 51 α-l-arabinofuranosidase from Penicillium purpurogenum: purification, properties and amino acid sequence A family 51 α-l-arabinofuranosidase from Penicillium purpurogenum: purification, properties and amino acid sequence](/preview/png/4357761.png)
چکیده انگلیسی
The soft rot fungus Penicillium purpurogenum secretes a wide variety of xylanolytic enzymes to the medium, among them three α-l-arabinofuranosidases. This work refers to arabinofuranosidase 2 (ABF 2). This enzyme was purified to homogeneity and characterized; it is a glycosylated monomer with a molecular weight of 70â000 and an isoelectric point of 5.3. When assayed with p-nitrophenyl α-l-arabinofuranoside (pNPAra) the enzyme followed Michaelis-Menten kinetics with a KM of 0.098 mm. The optimum pH is 5 and the optimal temperature 60 °C. ABF 2 showed weak activity on natural polymeric substrates, such as sugar beet arabinan, debranched arabinan, and arabinoxylan. These results, together with its low KM (pNPAra) and its activity towards short arabinooligosaccharides, suggest that the enzyme belongs to the exo α-l-arabinosyl hydrolases not active on polymers. The abf2 gene and its cDNA were sequenced, and the gene was found to possess seven introns. The mature protein is 618 amino acids long with a calculated molecular weight of 67â212. Amino acid sequence alignments show that the enzyme belongs to family 51 of the glycosyl hydrolases, although it differs in some properties from other enzymes of this family.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mycological Research - Volume 112, Issue 8, August 2008, Pages 933-942
Journal: Mycological Research - Volume 112, Issue 8, August 2008, Pages 933-942
نویسندگان
Macarena Fritz, MarÃa Cristina Ravanal, Christophe Braet, Jaime eyzaguirre,