کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4357906 1300113 2007 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Inoculum production and long-term conservation methods for cucurbits and tomato powdery mildews
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
Inoculum production and long-term conservation methods for cucurbits and tomato powdery mildews
چکیده انگلیسی

The behaviour of cucurbit powdery mildews (Podosphaera xanthii and Golovinomyces cichoracearum) and tomato powdery mildew (Oidium neolycopersici) infesting detached cotyledons of Lagenaria leucantha cv. ‘Minibottle’ was studied in order to develop an easy culture method for pure inoculum production. High spore production was found with a combination of mannitol (0.1 m), sucrose (0.02 m) and agar (8 g l−1) in the cotyledon survival medium. Sporulation on cotyledons and viability of conidia were affected by the age of culture for the three species of powdery mildew tested. The age of cotyledons had also an impact of the spore production. This method was used to produce large amounts of inoculum for P. xanthii, G. cichoracearum and O. neolycopersici and enable the development of other species of powdery mildew like Leveillula taurica. Freezing conidia in liquid nitrogen enabled the long-term conservation of P. xanthii without any loss of virulence. The same method was unsuccessful with G. cichoracearum, and L. taurica and partly successful with O. neolycopersici.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mycological Research - Volume 111, Issue 6, June 2007, Pages 740–747
نویسندگان
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