کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4358216 1300133 2006 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Isolation and characterisation of Beauveria bassiana isolates from phylloplanes of hedgerow vegetation
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
Isolation and characterisation of Beauveria bassiana isolates from phylloplanes of hedgerow vegetation
چکیده انگلیسی

A leaf imprinting technique combined with a selective medium was used to document the natural occurrence of Beauveria bassiana on phylloplanes of typical hedgerow plants (grasses, stinging nettle and hawthorn) in May, July and September in a hedgerow in Denmark. The density of B. bassiana (as measured by numbers of colony forming units) was greatest in September and on lower nettle leaves. B. bassiana was isolated from phylloplanes in a different hedgerow the following year and a similar picture of occurrence was found. Genetic diversity of selected in vitro isolates were characterised by Universally Primed (UP) PCR, and 13 distinguishable banding patterns were found at the two localities. Of these, four were shared between the field sites and all plant species harboured isolates of B. bassiana with at least two different banding patterns. The isolation method described represents a valuable tool for studying naturally occurring B. bassiana and for rapid isolation of indigenous strains of the fungus for future development of biocontrol agents. The significance of the findings for the life-cycle of B. bassiana is discussed.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mycological Research - Volume 110, Issue 2, February 2006, Pages 188–195
نویسندگان
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