کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4362715 | 1616250 | 2015 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Location and stoichiometry of the protease CspB and the cortex-lytic enzyme SleC in Clostridium perfringens spores
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Location and stoichiometry of the protease CspB and the cortex-lytic enzyme SleC in Clostridium perfringens spores Location and stoichiometry of the protease CspB and the cortex-lytic enzyme SleC in Clostridium perfringens spores](/preview/png/4362715.png)
چکیده انگلیسی
The protease CspB and the cortex-lytic enzyme SleC are essential for peptoglycan cortex hydrolysis during germination of spores of the Clostridium perfringens food poisoning isolate SM101. In this study, Western blot analyses were used to demonstrate that CspB and SleC are present exclusively in the C. perfringens SM101 spore coat layer fraction and absent in the lysate from decoated spores and from the purified inner spore membrane. These results indicate why decoating treatments greatly reduce both germination and apparent viability of C. perfringens spores in the absence of an exogenous lytic enzyme. In addition, quantitative Western blot analyses showed that there are approximately 2000 and 130,000 molecules of CspB and pro-SleC, respectively, per C. perfringens SM101 spore, consistent with CspB's role in acting catalytically on pro-SleC to convert this zymogen to the active enzyme.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Microbiology - Volume 50, September 2015, Pages 83-87
Journal: Food Microbiology - Volume 50, September 2015, Pages 83-87
نویسندگان
Saeed Banawas, George Korza, Daniel Paredes-Sabja, Yunfeng Li, Bing Hao, Peter Setlow, Mahfuzur R. Sarker,