کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4362972 1301529 2013 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fast identification of wine related lactic acid bacteria by multiplex PCR
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Fast identification of wine related lactic acid bacteria by multiplex PCR
چکیده انگلیسی

The microflora of must and wine consists of yeasts, acetic acid bacteria and lactic acid bacteria (LAB). The latter group plays an important role for wine quality. The malolactic fermentation carried out by LAB leads to deacidification and stabilisation of wines. Nevertheless, LAB are often associated with wine spoilage. They are mainly responsible for the formation of biogenic amines. Furthermore, some strains produce exopolysaccharide slimes, acetic acid, diacetyl and other off-flavours. In this context a better monitoring of the vinification process is crucial to improve wine quality. Moreover, a lot of biodiversity studies would also profit from a fast and reliable identification method.In this study, we propose a species-specific multiplex PCR system for a rapid and simultaneous detection of 13 LAB species, frequently occurring in must or wine: Lactobacillus brevis, Lb. buchneri, Lb. curvatus, Lb. hilgardii, Lb. plantarum, Leuconostoc mesenteroides, Oenococcus oeni, Pediococcus acidilactici, P. damnosus, P. inopinatus, P. parvulus, P. pentosaceus and Weissella paramesenteroides.


► Development of species-specific primers for wine related lactic acid bacteria.
► Fast identification of certain lactic acid bacteria by multiplex PCR in must or wine.
► Possible application for wine monitoring, microbial quality control and biodiversity studies.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Microbiology - Volume 33, Issue 1, February 2013, Pages 48–54
نویسندگان
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