Comparison of heat stress responses of immobilized and planktonic Salmonella enterica serovar Typhimurium

► Immobilisation of Salmonella changes the heat stress response compared to planktonic growth. ► A combination of immobilisation and heat stress induces flagella and SPI1 gene expression. ► A combination of immobilisation and heat stress induce the invasion ability of Salmonella to HeLa cells.