کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4363118 1301540 2011 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of the growth environment on the strain variability of Salmonella enterica kinetic behavior
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Effect of the growth environment on the strain variability of Salmonella enterica kinetic behavior
چکیده انگلیسی

Intra-species variability of microbial growth kinetic behavior is an event with important implications for food safety research. Aiming at the evaluation of the growth variability among Salmonella enterica strains as affected by the growth environment, the kinetic behavior of 60 isolates of the pathogen was assessed at 37 °C in tryptone soy broth of different pH values (4.3–7.0) and NaCl concentrations (0.5–6.0%). Maximum specific growth rate (μmax) values corresponding to each strain and growth condition were estimated by means of absorbance detection times of serially decimally diluted cultures using the automated turbidimetric system Bioscreen C. A total of 9600 optical density curves were generated for the strains and the growth conditions tested. The variability of μmax among the S. enterica strains was important and greater than that observed within the strains (i.e. among replicates). Moreover, strain variability increased as the growth conditions became more stressful both in terms of pH and NaCl. The coefficient of variation of μmax among the tested strains at pH 7.0–0.5% NaCl was 6.1%, while at pH 4.3–0.5% NaCl and pH 7.0–6.0% NaCl was 11.8% and 23.5%, respectively. Beyond the scientific interest in understanding strain variability, the findings of this study should be useful in strain selection for exploitation in food safety challenge studies as well as in incorporating strain variability in predictive microbiology and microbial risk assessment.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Microbiology - Volume 28, Issue 4, June 2011, Pages 828–837
نویسندگان
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