کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4363391 | 1301554 | 2010 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Influence of storage temperature on gene expression and virulence potential of Listeria monocytogenes strains grown in a salmon matrix
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Influence of storage temperature on gene expression and virulence potential of Listeria monocytogenes strains grown in a salmon matrix Influence of storage temperature on gene expression and virulence potential of Listeria monocytogenes strains grown in a salmon matrix](/preview/png/4363391.png)
چکیده انگلیسی
Little is understood about the impact of environmental conditions on the virulence plasticity of Listeria monocytogenes strains grown in food. In this report, we monitored changes in the virulence properties of one high virulent (CCUG 3998) and one low virulent (442) L. monocytogenes strains grown on raw salmon (Salmo salar L.). The effect of temperature exposures (0 °C, 4 °C and 20 °C) on the expression levels of virulence genes (hlyA, actA, inlA and prfA), invasion into Caco-2 cells and in vivo mouse infection was analysed. Our results showed that L. monocytogenes virulence genes are differentially expressed when salmon is stored at different temperatures. Of the four virulence genes, the transcript levels for inlA were strongly affected, which correlated with the strain's virulence capacity as assessed by Caco-2 cells. In contrast to CCUG 3998, the virulence of strain 442 was altered with tested conditions. This strain maintains its low virulence status as far as salmon is stored at lower temperatures, but increases its virulence at higher temperatures. These results lead to the indication that exposure to abuse temperature conditions might influence the virulence potential of low pathogenic L. monocytogenes strains in salmon.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Microbiology - Volume 27, Issue 6, September 2010, Pages 795-801
Journal: Food Microbiology - Volume 27, Issue 6, September 2010, Pages 795-801
نویسندگان
Samuel Duodu, Arne Holst-Jensen, Taran Skjerdal, Jean-Michel Cappelier, Marie-France Pilet, Semir Loncarevic,