کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4383152 1304251 2008 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determining species diversity of microfungal communities in forest tree roots by pure-culture isolation and DNA sequencing
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک بوم شناسی، تکامل، رفتار و سامانه شناسی
پیش نمایش صفحه اول مقاله
Determining species diversity of microfungal communities in forest tree roots by pure-culture isolation and DNA sequencing
چکیده انگلیسی
Pure-culture isolation from roots was compared with transformation of total DNA from roots followed by sequence analysis of ITS 1/2 rDNA of representative clones as methods for determining the abundance and composition of microbiota in roots of Betula pendula, Fagus sylvatica, Larix decidua, Prunus serotina and Quercus petraea. The results from the two methods differed greatly, with no overlap between the taxa identified. Pure-culture isolation revealed greater species diversity (47 taxa), the most frequent fungi being Ascomycota, including Penicillium spp., Phialocephala fortinii, Pochonia bulbillosa, Sesquicillium candelabrum and Trichoderma spp. Transformation of total DNA and sequencing revealed less diversity (22 taxa), the most frequent taxa being Basidiomycota, including Coprinus fissolanatus and Mycena spp., and Ascomycota, including Podospora-Schizothecium spp., Helgardia anguioides and Microdochium sp. Communities characterized by either method showed slightly greater fungal diversity and less species dominance on F. sylvatica than on roots of other trees, whilst DNA sequencing showed least diversity and greatest species dominance on Q. petraea.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Applied Soil Ecology - Volume 40, Issue 1, September 2008, Pages 44-56
نویسندگان
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