Development of novel enzymatic bioremediation process for textile industry effluents through response surface methodology

Crude ligninolytic enzymes extract from Schyzohyllum commune IBL-06 having high activities of LiP (2186.02 U mL−1), MnP (1676.75 U mL−1) and laccase (259.07 U mL−1) was used for bioremediation of textile industry effluents. Response Surface Methodology (RSM) was adopted using D-optimal design to optimize the effluent decolorization process. The effects of different mediators on effluent decolorization were also investigated under preoptimized conditions. The optimum conditions for maximum decolorization of the effluents collected from Khyber Textile (KHT), Ishaq Textile (IST), Kalash Textile (KAT) and Masood Textile (MAT) industries of Faisalabad, Pakistan were: initial pH 4.5; temperature, 35 °C; effluent concentration, 180 ppm and veratryl alcohol (mediator), 1 mM. It was noted that values of water quality parameters including pH, color, biochemical oxygen demand (BOD), chemical oxygen demand (COD), total suspended solids (TSS), total organic carbon (TOC) and formaldehyde for enzyme treated effluents were within permissible limits set by the National Environmental Quality Standards (NQS). Analysis of peaks in HPLC profiles for treated and untreated effluents confirmed the degradation of residual dyes by ligninolytic enzymes extract of S. commune IBL-06.