Effects of sulfadiazine-contaminated fresh and stored manure on a soil microbial community

► SDZ-contaminated manure affects soil microbial activities and structure on a mid-term of 8 weeks. ► The N cycle is highly sensitive to contamination with sulfonamide antibiotics. ► Contaminated, 6 months-stored manure but not fresh manure affected structural diversity (PLFA). ► Conventional manure storage seems unsuited to reduce risks from sulfonamide antibiotics in soil.