کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4395851 | 1618441 | 2012 | 8 صفحه PDF | دانلود رایگان |

We describe a strategy to identify stress-responsive genes in corals by means of high-coverage cDNA expression profiling (HiCEP). In this study, the effects of thermal and chemical (DCMU and TBT-Cl) stresses on Acropora tenuis juvenile polyps were examined, and 19 genes were identified as stress-responsive genes. Of these, seven had sequences significantly similar to sequences of known proteins. We quantified the expression levels of these seven using real-time PCR. In corals, the genes encoding heat shock protein 90, calreticulin, methionine adenosyltransferase, and aquaglyceroporin showed differential stress-related expression patterns. In the endosymbiotic algae associated with the corals, genes coding for DnaJ-like protein and chloroplast light-harvesting protein were identified as stress-responsive genes. The only gene showing increased expression in response to all three stresses (thermal and both chemical stresses) was the coral gene coding for the oxidative stress-responsive protein, suggesting that oxidative stress generally occurs in each stress-exposed polyp.
► The effects of thermal and chemical stresses on coral juveniles were examined.
► We identified 19 genes whose expression changed by stress treatment.
► Some chaperon function proteins were identified from coral and the symbiont.
► Only oxidative stress-responsive protein was generally expressed by each stressor.
Journal: Journal of Experimental Marine Biology and Ecology - Volumes 430–431, 1 November 2012, Pages 17–24