کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4396969 1305856 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Expression of Hsp70, Na+/K+ ATP-ase, HIF-1α, IL-1β and TNF-α in captive Pacific bluefin tuna (Thunnus orientalis) after chronic warm and cold exposure
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
پیش نمایش صفحه اول مقاله
Expression of Hsp70, Na+/K+ ATP-ase, HIF-1α, IL-1β and TNF-α in captive Pacific bluefin tuna (Thunnus orientalis) after chronic warm and cold exposure
چکیده انگلیسی

Bluefin tuna (Thunnus spp.) are recently evolved teleosts with exceptional physiological and morphological specializations, whose aquaculture has become an increasing sector of the bluefin fishery. We studied the molecular expression of target gene transcripts in respect to experimental chronic cold and warm exposure in Pacific bluefin tuna liver, spleen, kidney and gill. The genes of interest were chosen as representative “biomarkers”, shown to change in fish exposed to temperature challenges: chaperon molecules (Hsp70); proteins engaged in active transport (Na+/K+ ATP-ase); oxygen-dependent genes (HIF-1α); and cytokines (IL-1β, TNF-α). In captive Pacific bluefin tuna, these genes showed constitutive expression in all organs examined. A seven-week chronic exposure to cold temperature resulted in shifts in expression levels of oxygen-dependent HIF transcripts in spleen and gill, and Hsp70 in spleen, while other gene transcripts remain unaffected. The results indicate that a long-term exposure to cold imposes physiological pressure on this endothermic fish species, especially on haemaotopoietic and gas-exchange organs. We suggest that these two genes can be considered as potential biomarkers for thermal shifts in captive bluefin.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Experimental Marine Biology and Ecology - Volume 374, Issue 1, 15 June 2009, Pages 51–57
نویسندگان
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