کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4489884 | 1317742 | 2011 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Rapid HPLC Method for Determination of 12 Isoflavone Components in Soybean Seeds Rapid HPLC Method for Determination of 12 Isoflavone Components in Soybean Seeds](/preview/png/4489884.png)
It is important to determine the isoflavone components by high-performance liquid chromatography (HPLC) for the molecular assistant selection of isoflavone in soybean. Based on the standard samples of 12 isoflavone components, the isoflavone components were analyzed using the determination of absorbance peaks method by HPLC. The results showed that there were different maximum ultraviolet (UV) absorbance for the aglycones of daidzein, glycitein, and genistein, which were at 250, 257, and 260 nm, respectively. A linear gradient elution of acetonitrile (13–30%) containing 0. 1% acetic acid as a mobile phase was applied on a YMC-C18 column at 35°C. The 12 isoflavone components were determined using the UV detector by HPLC. We concluded that this is a rapid and precise method which adapted to determine the large numbers of samples with microanalysis.
Journal: Agricultural Sciences in China - Volume 10, Issue 1, January 2011, Pages 70-77