Antioxidant Activity of Pigment Extracted from Green-Wheat-Bran

The anthocyanin pigment extracted from green-wheat-bran was studied to identify its antioxidant activity. The antioxidant activities of the pigment were evaluated by anti-lipid peroxidation, total antioxidant activity (TAA), superoxide anion radical scavenging activity (SARSA), active oxygen scavenging activity (AOSA), and DPPH (1,1-diphenyl-2 picrylhydrazyl free radical) radical scavenging activity. The results showed that the pigment had higher antioxidant activity and TAA,. SARSA, AOSA and DPPH scavenging activities at a certain concentration than Vc (antiscorbutic vitamin, vitamin C), and the capacity increased with the increase of pigment concentration. Its TAA was 51.06 U mL−1, 1.73 times of Vc, and SARSA. 18 025.21 U mL−1, 2.26% higher than Vc, and AOSA 3 776.31 U mL−1, 1.24 times of Vc. As to the DPPH scavenging activity of the pigment, there was a trend that higher concentration performed higher activity significantly improved with the company of Vc. The pigment showed significant antioxidant activities evaluated by different assays. Results will provide a better understanding on antioxidant activity of green wheat and allow the screening or breeding of green wheat varieties with higher antioxidant activity for food processing.