1B Specific LMW-GS Primers Cloned a 1D Located Gene from Wheat Cultivar Xiaoyan 22

In the present study, one unique low-molecular-weight glutenin subunit (LMW-GS) gene, LMWXY22-2 (GenBank no. FJ028810), was isolated from wheat cultivar Xiaoyan 22 (Triticum aestivum L.) by a pair of genomic specific PCR primers for 1B chromosome. Sequence analysis revealed that LMWXY22-2 was composed of 1 364 bp nucleotides, including a 317 bp promotion region and a 1 047 bp coding region which could be translated into a mature protein of 349 amino acids. In spite of a few minor mutations, the sequence of 5′ untranslated region (UTR), the coding region, the deduced N- and C- terminus comparisons indicated that LMWXY22-2 belonged to the reported subunits of LMW-m type and type III group 5, respectively. Inner gene markers for 1D chromosome together with the phylogenetic analysis revealed that this gene was classified into Glu-D3, which was not in agreement with the 1B locus-specific primers for LMW genes completely.