کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4490336 | 1317764 | 2009 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Research of Genetic Diversity in Seven Kobresia by AFLP in Tibetan Plateau Research of Genetic Diversity in Seven Kobresia by AFLP in Tibetan Plateau](/preview/png/4490336.png)
This work analyzed the genetic diversity of Kobresia accessions at the molecular level, and further obtained the necessary information for breeding and germplasm evaluation. Genomic DNA of Kobresia was amplified with four E+3 and M+3 primer combinations with AFLP (amplified fragment length polymorphism). AFLP analysis produced 164 scorable bands, of which 154 (93.96%) were polymorphic. The mean Nei's gene diversity index (H) was 0.2430, and the Shannon's information index (I) was 0.4012, indicating the abundant genetic diversity of Kobresia. The 11 Kobresia accessions from Tibetan Plateau, China, can be classified into five groups after cluster analysis based on the UPGMA (unweighted pair group method arithmetic average) method. In general, there was abundant genetic diversity among Kobresia accessions resources, and the genetic coefficient was unrelated to their geographic latitude. Natural habitats influenced genetic differentiation of Kobresia.
Journal: Agricultural Sciences in China - Volume 8, Issue 8, August 2009, Pages 994-999