کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4490450 | 1317770 | 2009 | 7 صفحه PDF | دانلود رایگان |
Process parameters on enzymatic hydrolysis and molecular weight (MW) distribution of collagen hydrolysates from Gadus morrhua skin were investigated. The optimal process parameters were obtained by the single-factor and orthogonal experiments. The molecular weight distribution of hydrolysates was determined using both Sephadex G25 partition and high speed liquid chromatography electricity spray mass spectrum (HPLC-ESI-MS). Collagen hydrolysates were first gained by an alkaline protease “alcalase” for 3 h at temperature (50°C), pH (10.0), substrate concentration (75 g L−1), and E/S (3%). The molecular weight distribution of collagen hydrolysates ranged from 300 to 1 500 Da, and most of peptides were under 1 200 Da. Sephadex G25 partition and HPLC-ESI-MS should be successfully employed to determine the molecular weight distribution of collagen hydrolysates.
Journal: Agricultural Sciences in China - Volume 8, Issue 6, June 2009, Pages 723-729