کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4490468 | 1317771 | 2009 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Studies on the Purification and Characterization of Soybean Esterase, and Its Sensitivity to Organophosphate and Carbamate Pesticides Studies on the Purification and Characterization of Soybean Esterase, and Its Sensitivity to Organophosphate and Carbamate Pesticides](/preview/png/4490468.png)
Soybean esterase, a cholinesterase-like enzyme, was purified by differential centrifugation firstly, then, ammonium sulfate precipitation, dialysis, and finally, DEAE-cellulose-32 ion-exchange chromatography after extracting it from soybean seeds with phosphate buffer (0.3 mol L−1, pH 7.0). The extract recovery rate of the purified enzyme was 8.18% and purification fold was 91.58. The soybean esterase appeared as two bands on the denaturing SDS-PAGE with molecular weights of 24 and 37.2 kDa, respectively, which proved that it is a dimer protein consisting of two subunits. The result of nondenaturing PAGE revealed that the soybean esterase is a single band with cholinesterase-like activity using α-naphthyl acetate as the substrate and fast blue B salt as coloring agent. The esterase showed very high sensitivity to 18 kinds of organophosphate pesticides and 6 kinds of carbamate pesticides with the lowest detective limits of 0.03125-0.0625 and 0.03125-0.25 mg kg−1, respectively, and can meet the demands of MRL specified by the most countries.
Journal: Agricultural Sciences in China - Volume 8, Issue 4, April 2009, Pages 455-463