کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4490545 | 1317775 | 2008 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular Detection and Characterization of Citrus Viroids
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Molecular Detection and Characterization of Citrus Viroids Molecular Detection and Characterization of Citrus Viroids](/preview/png/4490545.png)
چکیده انگلیسی
Total RNA was isolated from the phloem of young shoots and retro-transcripted to cDNA by reverse transcriptase- polymerase chain reaction (RT-PCR) with specific primers; the amplified cDNA fragments were sequenced for identification of subtypes. In this study, an effective RT-PCR technique was established for detecting citrus viroids, and three citrus viroids were recognized, namely, CEVd, CVd-II, and CVd-III. The latter two were identified for the first time in China. The sequences of CVd-II were different from all the three subtypes reported in the GenBank and seemed to be a new subtype. CVd-IIIb detected in the present study could be further developed as a dwarfing agent.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Agricultural Sciences in China - Volume 7, Issue 11, November 2008, Pages 1333-1340
Journal: Agricultural Sciences in China - Volume 7, Issue 11, November 2008, Pages 1333-1340