Identification of AFLP Markers Linked to Lr19 Resistance to Wheat Leaf Rust

AFLP analyses were carried out on Thatcher, 23 near-isogenic lines and F2 generation of TcLr19 x Thatcher, to develop molecular markers for gene Lr19 resistance to wheat leaf rust. Seven markers linked to Lr19 resistance trait were obtained, which were P-AGT/M-GAG289bp (3.3 cM), P-ACA/M-GGT102bp (4.1 cM), P-ACA/M-GGT106bp (4.1 cM), P-AAC/M-CAG123bp (4.9 cM), P-AAC/M-GGT203bp (5.0 cM), P-ACA/M-GGT290bp (5.7 cM), and P-ATC/M-GAG293bp (9.6 cM). All of these specific fragments were isolated from the polyacrylamide gels, reamplified, cloned, and sequenced. The research may facilitate genetic mapping, physical mapping, and the eventual cloning of Lr19.