کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4490954 1317798 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Structure and Expression of Several Putative Cdc42-Interacting Proteins in Magnaporthe grisea
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
Structure and Expression of Several Putative Cdc42-Interacting Proteins in Magnaporthe grisea
چکیده انگلیسی

MgCdc42 (Cdc42 in Magnaporthe grisea), with high homology to ScCdc42 (Cdc42 in Saccharomyces cerevisiae), has been demonstrated to involve in the morphogenesis and infection process. To further understand the signaling network, the putative MgCdc42-interacting proteins were analyzed. ScCdc42-interacting protein sequences were first used to BLAST against the M. grisea genome database to retrieve their corresponding analogs. Subsequently, conserved domains of these proteins were compared and expression patterns of their encoding genes in different MgCdc42 mutation states were analyzed by semiquantitative RT-PCR. All retrieved analogs of ScCdc42-interacting proteins from the M. grisea database have conserved domains as those in S. cerevisiae. Expression of their encoding genes increased in MgCdc42CA mutant and decreased in MgCdc42KO mutant. However, MgBeml, Chml, and MgGicl in MgCdc42DN mutant had the same expression level as that in the wild type, although MgBem4, MgBoi2, MgCdc24, MgGic2, MgRgal, and Mst20 had decreased expression level, as expected. Overall, it is concluded that there may exist a similar Cdc42 signal pathway in M. grisea as in S. cerevisiae and MgCdc42 plays a key role in the pathway.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Agricultural Sciences in China - Volume 5, Issue 10, October 2006, Pages 780-786