کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4490996 | 1317801 | 2006 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Synthesis and Identification of Artificial Antigen for Imidacloprid
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
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چکیده انگلیسی
This study reported that a hapten of imidacloprid, 1-[(6-Carboxylethylthio-3- pyridinyl)methyl)-N-nitro-imidazolidinimine was synthesized using technical material of imidacloprid to react with 3-mercaptopropionic acid in hot alkaline solution. The hapten was conjugated to bovine serum albumin (BSA) and ovalbumin (OVA) to form the immuno-antigens and the coating antigen, respectively. The antibody with high titer (2.56 × 104) was obtained after immuning rabbits. The results showed that the antibody had a high affinity to imidacloprid tested by enzyme-linked immunosorbent assay (ELISA), which IC50 and IC20 were 20.7 and 1.1 μg L−1; and the cross reactibilities to those compounds related with imidacloprid were less than 1.4%.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Agricultural Sciences in China - Volume 5, Issue 4, April 2006, Pages 307-312
Journal: Agricultural Sciences in China - Volume 5, Issue 4, April 2006, Pages 307-312