کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4495558 | 1318768 | 2011 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Establishment and Optimization of a Rapid Molecular Marking Method for Developing CAPS Molecular Markers in Soybean
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Establishment and Optimization of a Rapid Molecular Marking Method for Developing CAPS Molecular Markers in Soybean Establishment and Optimization of a Rapid Molecular Marking Method for Developing CAPS Molecular Markers in Soybean](/preview/png/4495558.png)
چکیده انگلیسی
In this study, a rapid molecular marking method, gspCAPS (genome sequence pool CAPS), was established to develop soybean CAPS markers. The bioinformatics tools were executed for analyzing the sequences of soybean SNP sites and selecting the appropriate restriction endonucleases. Meanwhile, DNA samples were mixed, and the primers were sequentially screened by PCR and enzyme digestion procedures. To evaluate this method, 61 pairs of primers were designed and polymorphisms were detected in nine soybean varieties. The results showed that the method remarkably improved its efficiency and characterized with advantages such as high efficiency, timesaving and low cost, which had a widespread prospect for applying in the future.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Northeast Agricultural University (English Edition) - Volume 18, Issue 1, March 2011, Pages 12-16
Journal: Journal of Northeast Agricultural University (English Edition) - Volume 18, Issue 1, March 2011, Pages 12-16