کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4499038 1319011 2007 25 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Mechanism of translation based on intersubunit complementarities of ribosomal RNAs and tRNAs
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
Mechanism of translation based on intersubunit complementarities of ribosomal RNAs and tRNAs
چکیده انگلیسی

A universal rule is found about nucleotide sequence complementarities between the regions 2653–2666 in the GTPase-binding site of 23S rRNA and 1064–1077 of 16S rRNA as well as between the region 1103–1107 of 16S rRNA and GUUCG (or GUUCA) of tRNAs. This rule holds for all species in the living kingdoms except for two protista mitochondrial rRNAs of Trypanosoma brucei and Plasmodium falciparum. We found that quite similar relationships for the two species hold under the assumption presented in the present paper. The complementarity between T-loop of tRNA and the region 1103–1107 of 16S rRNA suggests that the first interaction of a ribosome with aminoacyl-tRNAEF-TuGTP ternary complex or EF-GGDP complex could occur at the region 1103–1107 of 16S rRNA with the T-loop–D-loop contact region of the ternary complex or the domain IV–V bridge region of the EF-GGDP complex. The second interaction should occur between the A-site codon and the anticodon loop or between the anticodon stem/loop of A-site tRNA and the tip of domain IV of EF-G. The above stepwise interactions would facilitate the collision of the region 1064–1077 of 16S rRNA with the region around A2660 at the α-sarcin/ricin loop of 23S rRNA. In this way, the universal rule is capable of explaining how spectinomycin-binding region of 16S rRNA takes part in translocation, how GTPases such as EF-Tu and EF-G can be introduced into their binding site on the large subunit ribosome in proper orientation efficiently and also how driving forces for tRNA movement are produced in translocation and codon recognition. The analysis of T-loops of all tRNAs also presents an evolutionary trend from a random and seemingly primitive sequence, as defined to be Y type, to the most developed structure, such as either 5G7 or 5A7 types in the present definition.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Theoretical Biology - Volume 245, Issue 4, 21 April 2007, Pages 644–668
نویسندگان
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