Comparison of Ephestia kuehniella eggs sterilization methods for Trichogramma rearing

• Ephestia kuehniella eggs sterilization methods were compared.
• UV irradiation, freezing at −15 °C and vitrification kill 100% of the embryos.
• UV and freezing were the most suitable sterilization methods for trichogramma rearing.

Mass production is necessary to ensure the availability of biological control agents for the suppression of target pests. Many rearing hosts need to be sterilized to prevent development. Host egg sterilization also allows their storage for a longer period. Ephestia kuehniella eggs are frequently used as hosts for Trichogramma parasitoïds but they must be sterilized to prevent larvae from emerging and eating the unhatched parasitized eggs. Three sterilization methods were examined: UV irradiation, freezing at −15 °C and vitrification (liquid nitrogen submersion). The dosage and exposure duration to provide egg sterilization were determined and then the suitability of hosts sterilized by the different methods were compared. E. kuehniella eggs abortion was achieved after 15 min by UV irradiation, 4 h by freezing at −15 °C and 30 s by vitrification. Vitrification resulted in significantly lower parasitoids production with a global emergence rate of 28.7%, compared to UV irradiation (75.1%), freezing at −15 °C (77.4%) and control (80.9%). Host eggs sterilization method did not affect sex-ratio, occurrence of malformation in adults, and female walking speed. Fecundity was significantly reduced in the females emerging from UV irradiated (37.2 offsprings) and vitrified (36.9 offsprings) eggs, compared to control (43.1 offsprings).

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