Screening of Brazilian Bacillus thuringiensis isolates active against Spodoptera frugiperda, Plutella xylostella and Anticarsia gemmatalis

The toxicity of a collection of 1400 isolates of Bacillus thuringiensis was assessed against the Lepidoptera Spodoptera frugiperda, Anticarsia gemmantalis and Plutella xylostella. Twenty seven isolates showed toxicity to the larvae of these insects with three isolates demonstrating significantly greater potency than the standard strain against Lepidoptera, B. thuringiensis serovar kurstaki HD1. These isolates were all found to produce bipyramidal crystals and major spore-associated protein bands of approximately 130 and 65 kDa, consistent with the detection of at least one cry1 and one cry2 family gene in each. The high level of insecticidal activity of these isolates makes them excellent candidates for further development for use in the field.