کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4519641 | 1322846 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Prickly pear polygalacturonase gene: cDNA cloning and transcript accumulation during ethylene treatment, cold storage and wounding
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Prickly pear polygalacturonase gene: cDNA cloning and transcript accumulation during ethylene treatment, cold storage and wounding Prickly pear polygalacturonase gene: cDNA cloning and transcript accumulation during ethylene treatment, cold storage and wounding](/preview/png/4519641.png)
چکیده انگلیسی
Polygalacturonase (PG; EC 3.2.1.6.9) has been the most widely studied cell wall hydrolase in fruit ripening. Degenerate oligonucleotides corresponding to conserved regions from reported sequences were used as primers for RT-PCR to amplify mRNA extracted from middle ripe fruit. Cloning and characterization of a cDNA OsPG showed a 282 bp product with a predicted sequence of 94 amino acids. The peptide exhibited a high identity with previously reported fruit PGs. Northern blot analysis of the messenger showed a 1.7 kb transcript induced during prickly pear ripening. It was found by Southern blot analysis that there is one copy of this gene. The OsPG mRNA expression is sensitive to ethylene, cold storage and wounding.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Postharvest Biology and Technology - Volume 44, Issue 3, June 2007, Pages 254–259
Journal: Postharvest Biology and Technology - Volume 44, Issue 3, June 2007, Pages 254–259
نویسندگان
Flor de Fátima Rosas-Cárdenas, María Leonor Valderrama-Cháirez, Andrés Cruz-Hernández, Octavio Paredes-López,