Multiple regeneration pathways in Spathoglottis plicata Blume - A study in vitro

► An efficient protocol for germination of immature seeds/embryos and mature seeds of Spathoglottis plicata was developed. ► A reliable and reproducible protocol for micropropagation has been devised. ► The in vitro raised plantlets were established in greenhouse with 80% survivability. ► This protocol can be extended to other economically important, rare and endangered orchids for mass propagation and conservation.