کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4527831 | 1625825 | 2014 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Establishment of cell suspension culture protocol for a seagrass (Halodule pinifolia): Growth kinetics and histomorphological characterization
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Establishment of cell suspension culture protocol for a seagrass (Halodule pinifolia): Growth kinetics and histomorphological characterization Establishment of cell suspension culture protocol for a seagrass (Halodule pinifolia): Growth kinetics and histomorphological characterization](/preview/png/4527831.png)
چکیده انگلیسی
Seagrasses are known for their secondary metabolites of great commercial value and this study provides the first report on the establishment of cell suspension culture for a seagrass. Basal medium supplemented with 3% sucrose, 2,4-dichlorophenoxy acetic acid (2,4-D - 2.0 mg lâ1) and N6-benzylaminopurine (BAP - 1.0 mg lâ1) was used for callus induction, and same medium with 2,4-D (2.5 mg lâ1) was used for establishing cell suspension cultures of Halodule pinifolia. A growth curve was observed with a lag phase until day 12, a log phase from day 12 to day 24, and a stationary phase from day 24 to day 36 with an average absolute cell growth rate of the suspension culture of 0.425 biomass dâ1 and a doubling time (td) of 51 h. The growth curve was derived with a Michaelis constant, Km (half the time required to attain the maximum cell cycle frequency) of 1.7597 hâ1 and a Vmax (maximum cell cycle frequency) of 0.0016 hâ1, and the relationship between the cell growth and substrate hydrolysis was significant (r2 = 0.9206). The histomorphological characterization of the established cell culture revealed different stages of the suspension of cells from a granular form of the suspension to a fine milky form. Consistent reproducibility of the growth pattern was observed in the suspension culture over one year so that this technique promises to be a step forward to the in vitro production of potential natural products from seagrass, since it is more convenient for large scale production in bioreactors.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Aquatic Botany - Volume 117, July 2014, Pages 33-40
Journal: Aquatic Botany - Volume 117, July 2014, Pages 33-40
نویسندگان
Pon. Subhashini, S. Raja, T. Thangaradjou,