Craniofacial abnormalities and altered wnt and mmp mRNA expression in zebrafish embryos exposed to gasoline oxygenates ETBE and TAME

► The developmental toxicity in an aquatic fish model was characterized for ETBE and TAME. ► ETBE and TAME disrupted normal craniofacial development in zebrafish embryos. ► Exposure to ETBE and TAME altered mRNA transcript levels of various Wnts and MMPs. ► ETBE and TAME are more toxic and target different organ systems than MTBE.