کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4529759 | 1625978 | 2012 | 8 صفحه PDF | دانلود رایگان |

Gallbladder bile from 2 fish species, mullet (Mugil liza) and tilapias (Tilapia rendalli), contain substantial matrix metalloproteinases (MMPs). Extensive purification studies were conducted in order to obtain workable samples for SDS-PAGE and zymography analysis. Proteinase activities were assayed by gelatin substrate zymography. Several protein bands were observed, corresponding to molecular weights of 200, 136, 43, 36, 34, 29, 23 and 14 kDa in mullet bile and 179, 97, 79, 61, 54, 45, 36, 33 and 21 kDa in tilapia bile. Specific inhibitor studies were conducted, in which MMPS were inhibited by EDTA and 1,10 phenanthroline, but not by serine and cysteine protease inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and transepoxysuccinyl-l-leucylamido-l-guanidino butane (E-64), confirming the proteinase identities as MMPs. Differences in proteinase expression were observed in fish from a contaminated and reference site. Some studies regarding MMPs in different fish tissues exist, however this is the first study conducted in fish bile, and their involvement in detoxification processes and organism protection against the effects of aquatic contaminants may be a possibility.
► This is the first study regarding MMP in fish bile; involvement in detoxification processes and contaminant protection is possible.
► Gallbladder bile from 2 fish species, mullets (Mugil liza) and tilapias (Tilapia rendalli), present substantial matrix metalloproteinases (MMPs).
► Extensive purification studies were conducted in order to obtain workable samples for SDS-PAGE and zymography analysis.
► Proteinase activities were assayed by gelatin substrate zymography.
► Inhibitor studies were conducted; MMPS were inhibited by EDTA and 1,10 phenanthroline, but not by serine and cysteine inhibitors.
► MMPs are differentially expressed in different environmental contamination situations.
Journal: Aquatic Toxicology - Volumes 110–111, April 2012, Pages 99–106