کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4530499 1324708 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cadmium tolerance in seven Daphnia magna clones is associated with reduced hsp70 baseline levels and induction
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
پیش نمایش صفحه اول مقاله
Cadmium tolerance in seven Daphnia magna clones is associated with reduced hsp70 baseline levels and induction
چکیده انگلیسی

The stress protein hsp70 is part of the intracellular alarm and repair system which enables organisms to counteract negative effects of toxicants on protein integrity. Under long-term selection pressure exerted by environmental pollution, in particular heavy metals, this system may be expected to play a major role in the course of local, microevolutionary events leading to the acquisition of toxicant resistance. Seven clones of Daphnia magna from different geographical regions were characterized regarding their sensitivity to Cd, their hsp70 expression, and Cd accumulation. In an acute immobilisation assay, the tested clones showed remarkable differences in their sensitivity to Cd. The highest EC50 values by far were obtained for the clone displaying lowest hsp70 expression. In general, hsp70 levels reflected the order of sensitivity to Cd among the seven clones reciprocally. Clonal variations in sensitivity and hsp70 expression could not be related to differential accumulation of Cd, though. In summary, the association of stress insensitivity with low hsp70 induction which has been exemplarily reported for populations of different invertebrates under strong selection pressure could be affirmed for a largely parthenogenetic species for the first time. Furthermore, our observation has serious consequences for the interpretation of toxicological assays using a single D. magna clone solely.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Aquatic Toxicology - Volume 94, Issue 2, 31 August 2009, Pages 131–137
نویسندگان
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