کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4530970 | 1324743 | 2007 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Peroxiredoxin 6 gene: A new physiological and genetic indicator of multiple environmental stress response in Pacific oyster Crassostrea gigas Peroxiredoxin 6 gene: A new physiological and genetic indicator of multiple environmental stress response in Pacific oyster Crassostrea gigas](/preview/png/4530970.png)
Peroxiredoxin 6 is a 1-cysteine peroxiredoxin involved in antioxidant processes. We characterised the full-length cDNA and genomic sequence of the gene encoding peroxiredoxin 6 (CgPrx6) in the Pacific oyster, Crassostrea gigas. A phylogenetic analysis of Prx6 sequences showed that the CgPrx6 segregates between vertebrate and invertebrate groups. We analysed the expression of mRNA coding for CgPrx6 using RT-PCR in gills and digestive gland of oysters sampled in different contaminated and reference estuaries of the Atlantic French coast. We also studied CgPrx6 exon 6 polymorphism by PCR-SSCP in the same populations. Our results showed that CgPrx6 gene expression was highly regulated in the estuaries showing differential contamination levels, as expression increased with pollution level. Polymorphism analysis revealed no significant allelic frequency variations between populations. However, heterozygote deficiency seems to occur in the most contaminated estuaries, suggesting a potential selective effect of environmental stress on heterozygote frequency. Finally, the use of CgPrx6 as a possible marker to monitor stress exposure in disturbed ecosystems is discussed.
Journal: Aquatic Toxicology - Volume 84, Issue 3, 15 October 2007, Pages 389–398