Evaluation of three field test kits to detect microcystins from a public health perspective

• Three microcystins kits were evaluated during cyanobacteria blooms.
• Performance of each kit was evaluated based on sensitivity, specificity, positive and negative predictive rate.
• Full comprehension of their scopes is imperative for kit selection and handling.
• End-user training is crucial for correct operation, interpretation and reporting.

Cyanobacteria blooms may present a public health concern in sources of drinking water and recreational water due to the production of toxins by some species, microcystins being the most commonly found. It is possible to detect microcystins using instrumental analyses and field test kits. While instrumental analysis methods are accurate, they are also costly, and in regions with a high incidence of blooms the time to report is lengthy (days). On the other hand, the use of commercially available test kits may provide quicker results at a lower cost. The purpose of this work was to evaluate three commercially available kits: the Immunochromatographic Strip Test for the Detection of Microcystins and Nodularins in Source Drinking Water at 1 μg/L (Abraxis strip test), the Abraxis Microcystin Tube Kit and the Envirologix QualiTube Kit. The evaluation of each kit focussed on the interpretation of the results by the end-user and the validity of a test kit was based on four indices: sensitivity, specificity, positive predictive rate (PPR) and negative predictive rate (NPR) (false positive/negative) based on the manufacturer's specifications. The results indicate that there are challenges in the visual interpretation of the results at levels close to the threshold value for each kit. The scope of each kit must be understood: free vs. total, qualitative vs. semiquantitative. For instance, the Envirologix Qualitube Kit does not provide a lysing agent, therefore it will underestimate the levels of total microcystin if a lysing step is not included. In the case of the Abraxis strip test, the kit provides information on the absence/presence of microcystin at a threshold value of 1 μg/L, but false positives were encountered.