کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4545371 1626939 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Monitoring a toxic bloom of Alexandrium minutum using novel microarray and multiplex surface plasmon resonance biosensor technology
ترجمه فارسی عنوان
نظارت بر شکوفه سمی الکساندریم مینیمم با استفاده از تکنولوژی میکروسکوپ رن میکروسیمر و چندکاره پانوسون رزونانس باسنسور
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
چکیده انگلیسی


• Measuring an Alexandrium minutum bloom from initiation to termination.
• Testing the MIDTAL microarray and multi SPR biosensor on field samples.
• Both the microarray and toxin detection platforms significantly correlated with LM counts.

Blooms of Alexandrium occur annually during the summer months in the North Channel of Cork Harbour on the south coast of Ireland. This study monitored an extensive bloom of the toxin producing Alexandrium minutum during the summer of 2011 with the use of the MIDTAL (Microarrays for the Detection of Toxic Algae) microarray and a prototype multiplex surface plasmon resonance (multi SPR) biosensor. Microarray signal intensities and toxin results from three testing platforms of the prototype multi SPR biosensor, commercial (CER) enzyme-linked immunosorbent assay (ELISA) and high performance liquid chromatography (HPLC) were compared against light microscopy counts. The main aim was to demonstrate the use of these methodologies to support national monitoring agencies by providing a faster and more accurate means of identifying and quantifying the harmful phytoplankton community and their toxins in natural water samples. Both the microarray signals and multi SPR biosensor results followed a significant trend with light microscopy results and both techniques indicated detection limits of <4000 cells of A. minutum in natural seawater samples.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Harmful Algae - Volume 32, February 2014, Pages 40–48
نویسندگان
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